Biotinylation of Cell Surface Proteins
نویسندگان
چکیده
منابع مشابه
Biotinylation and Purification of Surface-exposed Helicobacter pylori Proteins.
Interactions between pathogenic bacteria and host cells are often mediated by proteins found on the surfaces of the bacteria. The Gram-negative bacterium Helicobacter pylori is predicted to produce at least 50 surface-exposed outer membrane proteins, but there has been relatively little progress in experimentally analyzing the cell-surface proteome of this organism. Herein, we describe in detai...
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Initial contact between the opportunistic fungal pathogen Candida albicans and host tissue occurs at the cell surface. Biotin derivatives have been used to label the cell-surface proteins of yeasts, with labelled proteins subsequently detected by avidin-reporter conjugates. Previous work has indicated that avidin can bind to C. albicans proteins in the absence of biotin, suggesting a possible h...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2012
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.170